Isolation of granulocytes and labelling with indium 111-oxine sulphate
Identifieur interne : 000795 ( Main/Exploration ); précédent : 000794; suivant : 000796Isolation of granulocytes and labelling with indium 111-oxine sulphate
Auteurs : RBID : ISTEX:259_1982_Article_BF00256461.pdfAbstract
The isolation of granulocytes from whole blood and labelling with Indium 111-oxine sulphate are described in detail. To isolate the cells a two-step method was used: (1) removal of the red blood cells by methyl cellulose-Ronpacon and (2) separation of the leucocyte-rich plasma with a double gradient (1077, 1097) technique. 111In-oxine sulphate was prepared by adding 111In-chloride to a buffered solution of oxine sulphate. The labelling of the granulocytes with 111In-oxine sulphate was done by incubation at room temperature for 5 min.
DOI: 10.1007/BF00256461
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Institut für Reaktorforschung, Würenlingen</wicri:regionArea><wicri:noRegion>Würenlingen</wicri:noRegion></affiliation></author><author><name>Kurt Deubelbeiss</name><affiliation wicri:level="1"><mods:affiliation>Hämatologisches Zentrallabor, Inselspital, Bern, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Hämatologisches Zentrallabor, Inselspital, Bern</wicri:regionArea><placeName><settlement type="city">Berne</settlement><region type="région" nuts="3">Canton de Berne</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="RBID">ISTEX:259_1982_Article_BF00256461.pdf</idno><date when="1982">1982</date><idno type="doi">10.1007/BF00256461</idno><idno type="wicri:Area/Main/Corpus">000E47</idno><idno type="wicri:Area/Main/Curation">000E47</idno><idno type="wicri:Area/Main/Exploration">000795</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="eng">The isolation of granulocytes from whole blood and labelling with Indium 111-oxine sulphate are described in detail. To isolate the cells a two-step method was used: (1) removal of the red blood cells by methyl cellulose-Ronpacon and (2) separation of the leucocyte-rich plasma with a double gradient (1077, 1097) technique. 111In-oxine sulphate was prepared by adding 111In-chloride to a buffered solution of oxine sulphate. The labelling of the granulocytes with 111In-oxine sulphate was done by incubation at room temperature for 5 min.</div></front></TEI><mods xsi:schemaLocation="http://www.loc.gov/mods/v3 file:///applis/istex/home/loadistex/home/etc/xsd/mods.xsd" version="3.4" istexId="fa08d2d05c23317f9c3a840b5c3a551c25ff9ec6"><titleInfo lang="eng"><title>Isolation of granulocytes and labelling with indium 111-oxine sulphate</title></titleInfo><name type="personal"><namePart type="termsOfAddress">Dr.</namePart><namePart type="given">Gertrude</namePart><namePart type="family">Pfeiffer</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Eidg. 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To isolate the cells a two-step method was used: (1) removal of the red blood cells by methyl cellulose-Ronpacon and (2) separation of the leucocyte-rich plasma with a double gradient (1077, 1097) technique. 111In-oxine sulphate was prepared by adding 111In-chloride to a buffered solution of oxine sulphate. 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